Targeted ablation of beta cells in the embryonic zebrafish pancreas using E. coli nitroreductase
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چکیده
منابع مشابه
Targeted ablation of beta cells in the embryonic zebrafish pancreas using E. coli nitroreductase
In order to generate a zebrafish model of beta cell regeneration, we have expressed an Escherichia coli gene called nfsB in the beta cells of embryonic zebrafish. This bacterial gene encodes a nitroreductase (NTR) enzyme, which can convert prodrugs such as metronidazole (Met) to cytotoxins. By fusing nfsB to mCherry, we can simultaneously render beta cells susceptible to prodrug and visualize M...
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ژورنال
عنوان ژورنال: Mechanisms of Development
سال: 2007
ISSN: 0925-4773
DOI: 10.1016/j.mod.2006.11.005